FastQCFastQC Report
Fri 26 Jun 2020
SRR9264354_S0_L001_I1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9264354_S0_L001_I1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324258554
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCGC11240167534.66421274425346No Hit
ACTTCTAT7189581622.172372976165185No Hit
GGCGAGTA7077971321.828171416566548No Hit
CAAATACG5183435715.98550180421763No Hit
CAAATACA53243721.642014353767827No Hit
CAAATACT12191530.3759817543626004No Hit
AATTCTAT8210620.25321213268594295No Hit
TTACGCGC7827020.24138206697856301No Hit
NTGCGCGC6037670.18619925135421408No Hit
CAAATACC5340400.16469573228282514No Hit
TTCCGCGC4099150.12641609448489677No Hit
NCTTCTAT3840660.11844436955084922No Hit
NGCGAGTA3827530.11803944576894647No Hit
TTGCGCCC3499890.10793516336966087No Hit
TTGCGCAC3491690.10768227875339258No Hit
TTTCGCGC3277190.10106718726686237No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)