FastQCFastQC Report
Fri 26 Jun 2020
SRR9264352_S0_L001_I1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9264352_S0_L001_I1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274238504
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGATAC8991166932.785939132748474No Hit
ACGCGGGT6115675822.300573080722465No Hit
GAATTCCA5386381119.64122842502087Illumina Small RNA Adapter 2 (100% over 8bp)
TTTACATG4901411117.872804250711635No Hit
AAATTCCA19145270.698124797238538No Hit
CGCAATAC8618110.31425601709087503No Hit
TTTACATA8422570.3071257273194577No Hit
AGCGATAC7825240.2853443220358291No Hit
ATTACATG7231900.2637084105447133No Hit
TAATTCCA6828100.2489840011671009No Hit
CGCTATAC6537730.2383957724623527No Hit
NGCGATAC5760330.21004818491862834No Hit
CCCGATAC4789880.17466110448152095No Hit
ACTCGGGT4226040.154100898975149No Hit
GAATTCAA4035790.14716350698879252No Hit
TTTACATC3893460.14197349909697582No Hit
NCGCGGGT3871900.1411873221128715No Hit
NAATTCCA3560780.12984245275783737No Hit
CTCGATAC3345160.12197995362460118No Hit
ACGCGGAT3261720.11893734659521042No Hit
GGCGATAC3241150.11818726957466193No Hit
CGCCATAC3224590.11758341563881927No Hit
ACCCGGGT3221350.11746527030354571No Hit
NTTACATG3160060.11523035437795418No Hit
ACGCGGGC3004110.1095436985026727No Hit
TTTACATT2869480.10463446810517898No Hit
TTTAAATG2863700.10442370266138849No Hit
CACGATAC2822600.10292500720467757No Hit
ACGCGGGA2753280.10039728046357778No Hit
CGCGATAA2752680.10037540169778639No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)