FastQCFastQC Report
Fri 26 Jun 2020
SRR9264351_S0_L001_I1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9264351_S0_L001_I1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214565379
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTGCG7267869433.872516777275614No Hit
GGACACGT5203517124.25142921123356No Hit
TACTCTTC4952884323.083333961347044No Hit
ATGAGAAA3438197716.02400963297998No Hit
CCTGTCCG5717820.2664838114447159No Hit
NCTGTGCG3658160.1704916243733804No Hit
CCTTTGCG3249100.15142703893529813No Hit
NGACACGT2528430.11783960729284289No Hit
GCACACGT2380560.11094800154129246Illumina Multiplexing Adapter 1 (100% over 8bp)
NACTCTTC2304940.1074236678229436No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)