FastQCFastQC Report
Fri 26 Jun 2020
SRR9264345_S0_L001_I1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9264345_S0_L001_I1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332110056
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTG9356606728.1732110514594Clontech Universal Primer Mix Short (100% over 8bp)
GCGAGAGT8825338826.573536815759653No Hit
AGTCCCAC7125205221.454349458180815No Hit
TACGTTCA7036630321.187646001300244No Hit
NTATAGTG7336900.2209177309584387No Hit
NCGAGAGT6911860.2081195638351884No Hit
NGTCCCAC5518720.16617142119900158No Hit
NACGTTCA5413600.16300620538873414No Hit
CTATAGTA4663880.14043176097022486No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)