July 2018

The many faces of RNA-seq

  • Different flavours:

    • mRNAseq

    • Targeted

    • Small RNA

    • Single Cell RNA-Seq

  • Discovery:

    • Transcripts

    • Isoforms

    • Splice junctions

    • Fusion genes

  • Differential expression:

    • Gene level expression changes

    • Relative isoform abundance

    • Splicing patterns

  • Variant calling

Workflow


Sources of Noise

Sources of Noise - Sampling Bias