29th October 2021
Single Cell RNAseq Analysis Workflow
Quality Control
We will now:
- Check the distribution of key quality metrics.
- Filter out genes that are not expressed in any cell
- Identify low-quality cells
- Filter and/or mark low quality cells
Quality Control
We will now:
- Check the distribution of key quality metrics.
- Filter out genes that are not expressed in any cell
- Identify low-quality cells
- Filter and/or mark low quality cells
Quality Control
Bioconductor R packages:
- scater
- DropletUtils
Orchestrating Single-Cell Analysis with Bioconductor Robert Amezquita, Aaron Lun, Stephanie Hicks, Raphael Gottardo